Project 04:
The contribution of cytosolic chaperones to the biogenesis of mitochondrial proteins
Johannes Buchner
E-Mail: johannes.buchner@tum.de
Phone: +49 89-289-13340
Chair of Biotechnology
Technical University of Munich
Doron Rapaport
E-Mail: doron.rapaport@uni-tuebingen.de
Phone: +49 7071-2974184
Interfaculty Institute of Biochemistry
University of Tübingen
Abstract
Newly synthesized nuclear encoded mitochondrial proteins are vulnerable to aberrant interactions in the cytosol. Therefore, cytosolic factors are required to counteract misfolding, aggregation, or degradation. In our project, we will address two main aims: (1) Define the chaperone interactome of different mitochondrial proteins en route to the mitochondria (led by the Rapaport group). (2) Reconstitute the interactions of the various (co)chaperones with mitochondrial precursor proteins in vitro (led by the Buchner group).
Project-relevant publications
Drwesh L, Heim B, Graf M, Kehr L, Hansen-Palmus L, Franz-Wachtel M, Macek B, Kalbacher H, Buchner J, Rapaport D (2022) A network of cytosolic (co)chaperones promotes the biogenesis of mitochondrial signal-anchored outer membrane proteins. Elife. 11:e77706.
Zhou J, Jung M, Dimmer KS, Rapaport D (2022) The multi-factor modulated biogenesis of the mitochondrial multi-span protein Om14. J Cell Biol. 2022 Apr 4;221(4):e202112030.
Vitali DG, Drwesh L, Cichocki BA, Kolb A, Rapaport D (2020) The biogenesis of mitochondrial outer membrane proteins show variable dependence on import factors. iScience. 23(1):100779.
Jores T, Lawatscheck J, Beke V, Franz-Wachtel M, Yunoki K, Fitzgerald JC, Macek B, Endo T, Kalbacher H, Buchner J, Rapaport D (2018) Cytosolic Hsp70 and Hsp40 chaperones enable the biogenesis of mitochondrial β-barrel proteins. J Cell Biol. 217(9):3091-3108.
Hoseini H, Pandey S, Jores T, Schmitt A, Franz-Wachtel M, Macek B, Buchner J, Dimmer KS, Rapaport D (2016) The cytosolic cochaperone Sti1 is relevant for mitochondrial biogenesis and morphology. FEBS J. 283(18):3338-52.
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